Product Description
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, often abbreviated as EDC or EDAC HCl, is a chemical reagent commonly used in biochemical and chemical research for several applications. Its primary use is as a coupling agent in the synthesis of amide bonds between carboxylic acids and aminesOptimal Use in Biotech and Pharmaceutical ApplicationsEDC HCl is valued for its efficiency in activating carboxyl groups to facilitate amide bond formation, making it indispensable in peptide synthesis, protein conjugation, and nucleic acid research. Its water solubility and minimal side-reactions make it a preferred choice for sensitive biotechnological and pharmaceutical workflows requiring high-purity reagents.
Safe Handling and Storage GuidelinesKeep EDC HCl in a tightly closed container, stored in a cool, dry, and well-ventilated environment to ensure stability. The compound is sensitive to moisture and light, which can lead to decomposition. Proper handling with appropriate PPE is essential due to its potential to irritate skin, eyes, and the respiratory tract.
FAQ's of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Hydrochloride (EDC HCl):
Q: How should 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Hydrochloride (EDC HCl) be stored to maintain stability?
A: EDC HCl must be kept in a tightly sealed container in a cool, dry, and well-ventilated area, away from direct light and moisture. These precautions help maintain its stability and prevent decomposition.
Q: What makes EDC HCl suitable for peptide synthesis and protein conjugation?
A: EDC HCl is an effective carboxyl-activating agent that promotes amide bond formation in aqueous solutions. Its water solubility and low side-reaction profile make it ideal for synthesizing peptides, coupling proteins, and crosslinking nucleic acids in biochemical research.
Q: When should EDC HCl be used in a chemical synthesis process?
A: EDC HCl is commonly used during the coupling stage, when activation of the carboxyl group is required for amide or peptide bond formation. It is particularly effective in aqueous-phase reactions, enhancing conjugation efficiency in various applications.
Q: Where is EDC HCl typically applied in research or industry?
A: This reagent is widely utilized in pharmaceutical, biotechnology, and academic research laboratories for peptide synthesis, protein and nucleic acid modification, and other conjugation reactions needing high-purity reagents.
Q: What are the benefits of using EDC HCl over other coupling reagents?
A: EDC HCl's main advantage is its high solubility in water, which facilitates biological and pharmaceutical syntheses. It minimizes unwanted side reactions and is easy to use, supporting efficient and cleaner coupling procedures.
Q: How should EDC HCl waste be disposed of after use?
A: Waste material and containers should be disposed of in compliance with local, regional, or national regulations for chemical waste. Consult your institution's hazardous waste disposal guidelines for specific procedures.
Q: What personal protective equipment (PPE) is recommended when handling EDC HCl?
A: It is advisable to use laboratory gloves, eye protection, and a lab coat when working with EDC HCl. The compound may cause irritation to skin, eyes, or the respiratory tract upon exposure.